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Membranes were washed three times in 1×PBS and positive signals were developed with enhanced chemiluminescence kit (Amershan Pharmacia Biotech, Little Chalfont Buckinghamshire, England)

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Absence of Growth-Promoting and Diabetogenic Effects Full-length growth hormone can stimulate: Linear growth Organ enlargement Elevation of IGF-1 Reduced insulin sensitivity in some contexts However, research demonstrated that the 176–191 fragment does not replicate these endocrine effects This distinction is critical to understanding why the fragment was investigated separately from full HGH